primer_id	primer_seq	hetero_id	hetero_seq	fr_dG_2s_ss_primer_hetero_hybr_min	fr_max_dG_ss_primer_hetero_hybr_3end_3_3_5	length_im	fw_max_dG_ss_primer_homo_hybr_3end_3_3_5	fw_max_dG_cs_primer_homo_hybr_3end_3_3_5	fw_prob_sngl_self_5end_1_3	fw_prob_sngl_self_3end_1_3	fwa_prob_sngl_tmpl60_annl_3end_1_3	fwa_prob_sngl_tmpl60_tb_5end_1_3	fwa_x_y_ratio_with_v_w_abs_0	fw_dG_2s_ss_primer_homo_hybr_min	fwa_dG_2s_sc_annl_3end_1_3	fw_dG_2s_sc_3end_1_6	fw_dG_2s_sc_3end_4_9	fw_dG_2s_sc_3end_7_12	fw_dG_2s_sc_5end_1_6	fwa_dG_2s_sc_c_tb_3end_1_6	fw_dG_2s_sc_5end_4_9	fw_dG_2s_sc_5end_7_12	fwa_dG_2s_sc_c_tb_5end_1_3	fw_dG_fd_s_primer_self_fold
example-0.Tr.237-18.195-10	GGGGGCGGCATCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.19301	3.46	120	5.01	-0.0	1.511891836	2.520811159	1.7741274535	2.25878493766	0.777777777777778	1.14975	-0.262209	2.1628	2.70779	2.00347	4.39655	4.39655	4.85439	2.37381	0.0339707	-1.146
example-0.Tr.237-18.195-14	GGGGGCGGCAGGGGTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.27165	3.46	124	5.01	5.48	2.440738622	2.5628777634	1.7741274535	2.31837378437	0.555555555555556	-1.60925	-0.262209	2.1628	2.70779	2.00347	4.39655	4.39655	4.85439	3.35385	0.385837	0.057
example-0.Tr.237-18.201-10	GGCAGGGGCATCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.27165	3.46	120	5.01	5.52	2.748639351	2.410422001	1.7741274535	2.15069066173	0.444444444444444	2.16769	-0.262209	2.1628	2.70779	2.00347	3.35385	3.35385	3.91929	2.37381	0.0339707	-0.05
example-0.Tr.237-18.201-14	GGCAGGGGCAGGAGTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.27165	3.46	124	5.01	8.63	2.713715276	2.5464360668	1.7741274535	1.53623020729	0.222222222222222	-0.357466	-0.262209	2.1628	2.70779	2.00347	3.35385	3.35385	3.91929	3.35385	-0.795736	0.606
example-0.Tr.237-18.207-10	GGCAGGAGCGTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	1.79137	3.46	120	5.01	-0.0	2.87532563	2.4678206991	1.7741274535	1.41739169441	0.111111111111111	0.752435	-0.262209	2.1628	2.70779	2.00347	3.35385	3.35385	2.73772	3.02675	0.843679	1.135
example-0.Tr.237-18.207-14	GGCAGGAGCGGAGCTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.789071	3.46	124	5.01	-0.0	2.9110227844	2.1972785802	1.77414299116	2.03450995745	0.157894736842105	3.89322	-0.262209	2.1628	2.70779	2.00347	3.35385	3.35385	2.73772	3.85664	0.204759	1.503
example-0.Tr.237-22.195-10	GGGGGCGGCATCCCTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.169328	5.89	124	5.01	-0.0	1.256976575	2.2201561793	1.77417269829	2.25766687278	0.454545454545455	0.169342	-0.262209	2.1628	2.70779	2.00347	4.39655	4.39655	4.85439	2.37381	0.0339707	-0.501
example-0.Tr.237-22.195-14	GGGGGCGGCAGGGGTCCCTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.181473	5.89	128	5.01	5.48	2.083992481	2.3289933503	1.77417269829	2.32196285519	0.272727272727273	4.6387	-0.262209	2.1628	2.70779	2.00347	4.39655	4.39655	4.85439	3.35385	0.385837	-0.265
example-0.Tr.237-22.201-10	GGCAGGGGCATCCCTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.169328	5.89	124	5.01	5.52	2.876833073	2.1646296641	1.77417269829	2.15046463635	0.181818181818182	2.37861	-0.262209	2.1628	2.70779	2.00347	3.35385	3.35385	3.91929	2.37381	0.0339707	0.942
example-0.Tr.237-22.201-14	GGCAGGGGCAGGAGTCCCTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.181473	5.89	128	5.01	8.63	2.821093024	2.2595362988	1.77417269829	1.5445183316	0.0	-2.35122	-0.262209	2.1628	2.70779	2.00347	3.35385	3.35385	3.91929	3.35385	-0.795736	1.399
example-0.Tr.237-22.207-10	GGCAGGAGCGTCCCTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.12777	5.89	124	5.01	-0.0	2.782550718	2.2671464174	1.77417269829	1.41998972227	0.0909090909090909	0.142314	-0.262209	2.1628	2.70779	2.00347	3.35385	3.35385	2.73772	3.02675	0.843679	0.061
example-0.Tr.237-22.207-14	GGCAGGAGCGGAGCTCCCTCTCTCCTCCACCCTTGG	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.181473	5.89	128	5.01	-0.0	2.9120811253	2.13930971497	1.77414216029	2.03272500901	0.304347826086957	2.23832	-0.262209	2.1628	2.70779	2.00347	3.35385	3.35385	2.73772	3.85664	0.204759	2.649
example-0.Tr.241-18.195-14	GGGGGCGGCAGGGGTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.181473	12.2	128	5.11	5.48	1.978111842	2.621524143	2.62951785254	2.32196285519	0.777777777777778	4.6387	0.385837	3.18504	2.27075	2.21722	4.39655	4.39655	4.85439	3.35385	0.385837	-0.326
example-0.Tr.241-18.201-10	GGCAGGGGCATCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.169328	12.75	124	3.66	5.52	2.877991619	2.947397928	2.62951785254	2.15046463635	0.666666666666667	2.37861	0.385837	3.18504	2.27075	2.21722	3.35385	3.35385	3.91929	2.37381	0.0339707	0.942
example-0.Tr.241-18.201-14	GGCAGGGGCAGGAGTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.181473	12.2	128	3.66	8.63	2.812681952	2.894699584	2.62951785254	1.5445183316	0.444444444444444	-2.61936	0.385837	3.18504	2.27075	2.21722	3.35385	3.35385	3.91929	3.35385	-0.795736	1.399
example-0.Tr.241-18.207-10	GGCAGGAGCGTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.12777	12.2	124	-0.0	-0.0	2.798883184	2.962527834	2.62951785254	1.41998972227	0.333333333333333	0.142314	0.385837	3.18504	2.27075	2.21722	3.35385	3.35385	2.73772	3.02675	0.843679	0.061
example-0.Tr.241-18.207-14	GGCAGGAGCGGAGCTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.181473	12.27	128	-0.0	-0.0	2.9077655107	2.9935098244	2.62965593993	2.03272500901	0.0526315789473684	2.23832	0.385837	3.18504	2.27075	2.21722	3.35385	3.35385	2.73772	3.85664	0.204759	2.649
example-0.Tr.241-18.213-10	AGCGGAGCAGTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	0.181473	12.2	124	-0.0	3.52	1.78061346	2.985972472	2.62951785254	2.06098037994	0.0	0.670109	0.385837	3.18504	2.27075	2.21722	3.85664	3.85664	2.56693	2.29965	-0.65686	0.421
example-0.Tr.241-22.195-14	GGGGGCGGCAGGGGCTACTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.27	132	4.15	5.48	2.41832911	2.807929153	2.6298949165	2.01763308167	0.409090909090909	1.14975	0.385837	3.18504	2.27075	2.21722	4.39655	4.39655	4.85439	3.35385	0.385837	0.585
example-0.Tr.241-22.201-10	GGCAGGGGCACTACTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.27	128	3.66	5.52	2.829004824	2.949607086	2.6298949165	2.15237309195	0.363636363636364	1.93389	0.385837	3.18504	2.27075	2.21722	3.35385	3.35385	3.91929	3.00397	0.0339707	0.083
example-0.Tr.241-22.201-14	GGCAGGGGCAGGAGCTACTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.27	132	3.66	9.38	2.679591459	2.874669727	2.6298949165	1.11821030037	0.136363636363636	3.46828	0.385837	3.18504	2.27075	2.21722	3.35385	3.35385	3.91929	3.35385	-0.795736	-0.046
example-0.Tr.241-22.207-10	GGCAGGAGCGCTACTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.27	128	-0.0	-0.0	2.765447645	2.981863729	2.6298949165	1.41304016691	0.0909090909090909	6.59415	0.385837	3.18504	2.27075	2.21722	3.35385	3.35385	2.73772	3.44121	0.843679	0.197
example-0.Tr.241-22.207-14	GGCAGGAGCGGAGCCTACTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.27	132	-0.0	-0.0	2.734865573	2.979300542	2.62989801087	2.0460533819	0.130434782608696	-1.88022	0.385837	3.18504	2.27075	2.21722	3.35385	3.35385	2.73772	3.85664	0.204759	0.117
example-0.Tr.241-22.213-10	AGCGGAGCAGCTACTCCCTCTCTCCTCCACCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.27	128	-0.0	3.52	1.73106848	2.997312953	2.6298949165	1.18397515733	0.272727272727273	2.60583	0.385837	3.18504	2.27075	2.21722	3.85664	3.85664	2.56693	3.17277	-0.65686	0.421
example-0.Tr.245-18.201-10	GGCAGGGGCACTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.24	128	-0.0	5.52	2.833617538	2.918684128	2.4852345388	2.15237309195	0.888888888888889	1.93389	0.161523	2.69448	1.73722	1.73722	3.35385	3.35385	3.91929	3.00397	0.0339707	0.083
example-0.Tr.245-18.201-14	GGCAGGGGCAGGAGCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.24	132	4.43	9.38	2.67309572	2.760120079	2.4852345388	1.11821030037	0.611111111111111	3.46828	0.161523	2.69448	1.73722	1.73722	3.35385	3.35385	3.91929	3.35385	-0.795736	-0.046
example-0.Tr.245-18.207-10	GGCAGGAGCGCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.24	128	-0.0	-0.0	2.77732465	2.754844224	2.4852345388	1.41304016691	0.555555555555556	6.59415	0.161523	2.69448	1.73722	1.73722	3.35385	3.35385	2.73772	3.44121	0.843679	0.197
example-0.Tr.245-18.207-14	GGCAGGAGCGGAGCCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.24	132	4.43	-0.0	2.74516734	2.712261037	2.48523583305	2.0460533819	0.263157894736842	-1.88022	0.161523	2.69448	1.73722	1.73722	3.35385	3.35385	2.73772	3.85664	0.204759	0.117
example-0.Tr.245-18.213-10	AGCGGAGCAGCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.24	128	-0.0	3.52	1.69118573	2.922489682	2.4852345388	1.18397515733	0.111111111111111	2.60583	0.161523	2.69448	1.73722	1.73722	3.85664	3.85664	2.56693	3.17277	-0.65686	0.421
example-0.Tr.245-18.213-14	AGCGGAGCAGCTCCCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.81017	12.24	132	-0.0	3.52	1.859084048	2.960951105	2.48523214422	1.13856814741	0.1	4.5181	0.161523	2.69448	1.73722	1.73722	3.85664	3.85664	2.56693	3.17277	0.161523	0.421
example-0.Tr.245-22.201-10	GGCAGGGGCACACCCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	1.72914	12.24	132	-0.0	5.52	2.861061256	2.98161836	2.48521973522	2.15205937894	0.545454545454545	1.0465	0.161523	2.69448	1.73722	1.73722	3.35385	3.35385	3.91929	2.83318	0.0339707	1.283
example-0.Tr.245-22.201-14	GGCAGGGGCAGGAGCACCCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.24	136	4.43	10.76	2.796418542	2.910515024	2.48521973522	1.12021084606	0.318181818181818	0.144438	0.161523	2.69448	1.73722	1.73722	3.35385	3.35385	3.91929	3.35385	-0.795736	0.588
example-0.Tr.245-22.207-10	GGCAGGAGCGCACCCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.24	132	-0.0	-0.0	2.6370662	2.71321179	2.48521973522	1.41424884883	0.272727272727273	2.84073	0.161523	2.69448	1.73722	1.73722	3.35385	3.35385	2.73772	3.72909	0.843679	-0.583
example-0.Tr.245-22.207-14	GGCAGGAGCGGAGCCACCCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.24	136	4.43	-0.0	2.68471615	2.801440175	2.48521861822	2.04381006059	0.0434782608695652	-4.12826	0.161523	2.69448	1.73722	1.73722	3.35385	3.35385	2.73772	3.85664	0.204759	0.117
example-0.Tr.245-22.213-10	AGCGGAGCAGCACCCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	12.24	132	-0.0	3.52	1.70142726	2.950483613	2.48521973522	1.8297345521	0.0454545454545455	-0.199246	0.161523	2.69448	1.73722	1.73722	3.85664	3.85664	2.56693	3.00199	-0.65686	0.421
example-0.Tr.245-22.213-14	AGCGGAGCAGCTCCCACCCTACTCCCTCTCTCCTCC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	3.08051	12.24	136	-0.0	6.67	1.74111979	2.9754692214	2.48521972346	1.13995595443	0.307692307692308	4.5181	0.161523	2.69448	1.73722	1.73722	3.85664	3.85664	2.56693	3.17277	0.161523	0.421
example-0.Tr.249-18.201-14	GGCAGGGGCAGGAGCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	4.97	136	-0.0	11.28	2.806419006	2.9576215284	2.67391289231	1.12021084606	0.833333333333333	0.144438	-0.795736	1.51291	2.44154	2.56891	3.35385	3.35385	3.91929	3.35385	-0.795736	0.588
example-0.Tr.249-18.207-10	GGCAGGAGCGCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	4.97	132	-0.0	-0.0	2.494890851	2.914509926	2.67391289231	1.41424884883	0.777777777777778	2.84073	-0.795736	1.51291	2.44154	2.56891	3.35385	3.35385	2.73772	3.72909	0.843679	-0.877
example-0.Tr.249-18.207-14	GGCAGGAGCGGAGCCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	4.97	136	-0.0	-0.0	2.619543809	2.90571874	2.67391163231	2.04381006059	0.473684210526316	-0.727213	-0.795736	1.51291	2.44154	2.56891	3.35385	3.35385	2.73772	3.85664	0.204759	0.117
example-0.Tr.249-18.213-10	AGCGGAGCAGCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.62326	4.97	132	-0.0	3.52	1.64052606	2.950714834	2.67391289231	1.8297345521	0.388888888888889	-0.199246	-0.795736	1.51291	2.44154	2.56891	3.85664	3.85664	2.56693	3.00199	-0.65686	0.421
example-0.Tr.249-18.213-14	AGCGGAGCAGCTCCCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	3.08051	4.97	136	-0.0	5.04	1.71174292	2.9729269703	2.67390635739	1.13995595443	0.0	4.5181	-0.795736	1.51291	2.44154	2.56891	3.85664	3.85664	2.56693	3.17277	0.161523	0.421
example-0.Tr.249-22.201-14	GGCAGGGGCAGGAGCTCCCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.72479	4.97	140	-0.0	9.38	2.752051596	2.965600274	2.67390635739	1.12342156482	0.5	4.96205	-0.795736	1.51291	2.44154	2.56891	3.35385	3.35385	3.91929	3.35385	-0.795736	0.226
example-0.Tr.249-22.207-10	GGCAGGAGCGCTCCCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.72479	4.97	136	-0.0	-0.0	2.451772935	2.95545835	2.67390635739	1.41518647793	0.454545454545455	7.67288	-0.795736	1.51291	2.44154	2.56891	3.35385	3.35385	2.73772	3.44121	0.843679	-0.907
example-0.Tr.249-22.207-14	GGCAGGAGCGGAGCCTCCCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	3.16123	4.97	140	-0.0	-0.0	2.623445708	2.9398071643	2.67390635739	2.04212368375	0.272727272727273	1.32858	-0.795736	1.51291	2.44154	2.56891	3.35385	3.35385	2.73772	3.85664	0.204759	0.117
example-0.Tr.249-22.213-10	AGCGGAGCAGCTCCCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	3.08051	4.97	136	-0.0	5.04	1.71174292	2.9729269703	2.67390635739	1.13995595443	0.0	4.5181	-0.795736	1.51291	2.44154	2.56891	3.85664	3.85664	2.56693	3.17277	-0.65686	0.421
example-0.Tr.249-22.213-14	AGCGGAGCAGCTCCCTCCCACCCTACTCCCTCTCTC	hetero.example.1	TTTATATATATATAAACTGGGGAGGTGAACGCGGGA	2.81017	4.97	140	-0.0	3.52	1.750739229	2.987173765	2.67390635739	1.14026233604	0.0	4.5181	-0.795736	1.51291	2.44154	2.56891	3.85664	3.85664	2.56693	3.17277	0.161523	0.421